Journal: Journal of Functional Morphology and Kinesiology
Article Title: Aerobic Exercise Induces Alternative Splicing of Neurexins in Frontal Cortex
doi: 10.3390/jfmk6020048
Figure Lengend Snippet: Continuous progressive (CP) aerobic exercise (AE) promotes physical performance and affects muscle fiber expression. ( A ) Schematic representation of CP exercise: CP program was carried out three times a week for a total time of 12 weeks. The training schedule is reported: 120” at 10 RPM, 120” at 12 RPM, 120” at 14 RPM, 120” at 16 RPM, 90” at 18 RPM, 90” at 20 RPM, 90” at 22 RPM, 90” at 24 RPM, 90” at 26 RPM, 60” at 28 RPM, 60” at 30 RPM, and 30” at 32 RPM. ( B ) Timeline of the training program. ( C ) Body weight of sedentary and trained mice at the end of exercise. ( D ) Histogram reporting the strength evaluated at the end of exercise, through a test using a force transducer as reported in materials and methods. ( E ) Histogram reporting resistance as number of falls in trained and sedentary mice. Resistance was evaluated by a test on the RotaRod at constant speed of 10 RPM considering the falls number/time (CP: 1st week 12.3 ± 9.1, 6th week 3.2 ± 3.6, and 12th week 2.0 ± 2.8). * p < 0.05. Error bars represent SD. ( F – I ) Relative expression of MyHC isoforms of quadriceps muscles from sedentary (S) and trained (T) mice. RT-qPCR analysis shows the expression levels (normalized to CyPA) of MyHC-I, MyHC-IIa, MyHC-IIb, and MyHC-IIx, in S e T mice. Statistical analysis was performed by unpaired Student’s t -test and two-way ANOVA (* p < 0.05; ** p < 0.01).
Article Snippet: The strength test was performed using a force transducer (AD Instruments, Ltd., Sydney, Australia; cat. no. MLT050/D).
Techniques: Expressing, Muscles, Quantitative RT-PCR